Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. This aptamer-based hot start does not require a separate high temperature incubation step to activate the enzyme. Taq DNA polymerase possesses a 5´→3´ polymerase activity^1,2,3 and a 5´ flap endonuclease activity^4,5.

It is supplied with 10X Standard Taq Reaction Buffer, which is detergent-free and designed to be compatible with existing assay systems.

Product Source

This product is related to the following categories:

Taq DNA Polymerase Products

This product can be used in the following applications:

Colony PCR,

Multiplex PCR,

Specialty PCR,

Routine PCR,

PCR